Surviving the deep freeze. Can plants help us make better bone grafts?

[xchrom]

http://www.elpais.com/articulo/sociedad/Surviving/the/deep/freeze/Can/plants/help/us/make/better/bone/grafts/elpepusoc/20120206elpepusoc_3/Tes

The challenge came from the Director of the Bone Bank at the University Hospital of Coimbra in Portugal. "Can you find a way of making the cartilage cells in bone grafts survive deep freezing?" The question was interesting, but we needed to understand the problem in more detail.

We found that bone banks use osteochondral grafts - pieces of bone covered with the cartilage layer that makes up the articulating surface of bone extremities - that can be collected from multi-organ donors (allografts) or, in certain circumstances, from the patient himself (autografts). Allografts are used to repair cartilage or bone extremities that have been damaged by trauma, degenerative and tumour diseases, or infections. Osteochondral allografts are carved from whole bones and are either transplanted fresh or cryopreserved by deep freezing for later transplantation. Cryopreserved allografts have the advantages of enabling size-matching of the allograft and the recipient, screening of infectious diseases of the donor, and quarantine of the allografts, and all of these are mandatory for safe transplantation. The resulting relaxation of time constraints also eases transportation of tissues to distant locations. The clinical outcome of cryopreserved versus fresh osteochondral allografts is poor, however, leading to a variable graft failure rate of 10-50%. Efficacy of the grafts seems to be proportional to their content of living chondrocytes, the cartilage cells. Just like bone cells, chondrocytes do not survive freezing/thawing, but unlike those, chondrocytes in the transplanted osteochondral allografts are not replaced by cells from the host. Since chondrocytes are essential to maintain cartilage integrity, any injury that kills even a small number can have severe consequences. The problem underlying the question of the Bone Bank Director was becoming clearer.

The next step was obvious: had the usual cryoprotective agents (CPA), such as glycerol and dimethyl sulfoxide (DMSO), been tested in osteochondral allografts? Were they effective? Several reports indicated that both compounds effectively protect chondrocytes removed from the articular cartilage from the damaging effects of freezing/thawing, but are almost worthless when used to freeze intact human articular cartilage. A major obstacle is the cytotoxicity of those CPA, which limits the duration, temperature of exposure, and range of concentrations usable. The need for less toxic, more effective CPA became obvious.

Where to find such an agent? We looked at compounds extracted from organisms naturally resistant to drought and cold. One such is arbutin, a compound extremely abundant in several frost- and drought-resistant plants, where it can account for as much as 25% of the leaves' dry weight. Arbutin had been shown to protect isolated cell structures from the damaging effects of both freezing/thawing and drying.

[thecrow]

1. Wow, that's acutally very interesting!

Thanks for posting

[izquierdista]

2. The rise of the manbages!

Human cabbage hybrids that wilt in the freezer and spring back into shape with the morning sun.